The U.S. Department of Agriculture (USDA), Agricultural Marketing Service (AMS), requests applications for the fiscal year (FY) 2022 USDA Regional Food Business Centers (Regional Food Centers). The Regional Food Centers will serve as the cornerstone of USDA’s development of local and regional supply chains. They will offer coordination, technical assistance, and capacity building support to small and mid-sized food and farm businesses, with the goal of creating a more resilient, diverse, and competitive food system. AMS will award cooperative agreements to regionally based partners to support USDA’s work on regional food supply chains. USDA is using approximately $400 million in funds authorized under the Consolidated Appropriations Act, 2021, Pub. L. No. 116—260, Division N, Title VII, Subtitle B, Section 751 to develop and implement the program, with approximately $360 million going directly to fund the Regional Centers.